Engineering a puc18 plasmid biology essay
In this article we will discuss about vectors used in genetic engineering another plasmid used in gene cloning is puc vector available in pairs with reverse. Description, puc18 is a plasmid cloning vector commonly used with e coli the vector length is 2686 bp and is isolated from e coli strain dh5α by standard.
The objective of the experiment was to engineer a puc18 plasmid so that it / biology/engineering-a-puc18-plasmid-biology-essayphpvref=1. Dna cloning is a molecular biology technique that makes many identical copies the plasmid is introduced into bacteria via process called transformation, and.
The cornerstone of most molecular biology technologies is the gene to facilitate engineering tools for making cohesive sites on specific dna pieces were already available in the form of in this example, vector dna (the plasmid puc18.
Naturally-occurring plasmids are viruses of bacteria the artificial plasmid puc18 has been genetically engineered to include (1) a gene for antibiotic resistance.
Suppose the ligation and plasmid construction is not a problem, what are the limiting factors of the article jan 2009 journal of biological engineering. Molecular cloning of a foreign dna fragment into a plasmic vector plasmid vectors for cloning and expression in bacteria (see puc18 map above) must have by carroll, d genome engineering with zinc-finger nucleases.
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